Colloidal gold is a commonly used labeling technology. It is a new type of immunolabeling technology that uses colloidal gold as a tracer marker for antigens and antibodies. It has unique advantages. In recent years, it has been widely used in various biological studies. Almost all immunoblotting techniques used clinically use their label. At the same time, it can be used in flow cytometry, electron microscopy, immunology, molecular biology, and even biochips.
Colloidal gold is made of chloroauric acid (HAuCl4) under the action of reducing agents such as white phosphorus, ascorbic acid, sodium citrate, tannic acid, etc., and can be polymerized into gold particles of a certain size, and it becomes a stable colloidal state due to static electricity Forming a negatively charged hydrophobic glue solution, it becomes a stable colloidal state due to static electricity, so it is called colloidal gold. Colloidal gold is negatively charged in a weak alkaline environment and can form a strong bond with the positively charged groups of protein molecules. Because this bond is electrostatic, it does not affect the biological characteristics of the protein.
Colloidal gold labeling is essentially a coating process in which proteins such as proteins are adsorbed onto the surface of colloidal gold particles. The adsorption mechanism may be the negative charge on the surface of the colloidal gold particles, which forms a strong bond with the positively charged groups of the protein due to electrostatic adsorption. Colloidal gold particles of various particle sizes, that is, different colors, can be easily prepared from chloroauric acid by reduction method. This spherical particle has a strong adsorption function for proteins and can be non-covalently combined with staphylococcal protein A, immunoglobulins, toxins, glycoproteins, enzymes, antibiotics, hormones, bovine serum albumin polypeptide conjugates, Therefore, it has become a very useful tool in basic research and clinical experiments.
Colloidal gold technology has the advantages of convenience, quickness, specific sensitivity, strong stability, no special equipment and reagents, and intuitive judgment of results. And broad application prospects.
Trunnano is one of the world's largest manufacturers of colloidal gold."We have been supplying colloidal gold hexaboride, ensuring product quality, and guaranteeing stable supply prices," said Rachel, sales manager at Luoyang Trunnano.Please contact Rachel if you are interested in colloidal gold.
Email: rachel@ihpa.net
Trunnano is one of the world's largest manufacturers of colloidal gold."We have been supplying colloidal gold hexaboride, ensuring product quality, and guaranteeing stable supply prices," said Rachel, sales manager at Luoyang Trunnano.Please contact Rachel if you are interested in colloidal gold.
Email: rachel@ihpa.net
